A comparative study between different methods to assess cell viability

Since the introduction of the trypan blue exclusion method of determining cell viability, there have been many technological advances to improve the reliability and reduce manual steps.

Ovizio’s digital holographic microscope solutions can overcome many of the issues associated with a range of methods. 

Manual cell counting

Advantage:

  • Low cost of equipment.

Disadvantages:

  • Low precision & accuracy.
  • Labor-intensive & dye-based.

Coulter counter

Advantage:

  • Label-free.

Disadvantages:

  • Unreliable viability determination.
  • Data interpretation is difficult.

Flow cytometer

Advantage:

  • Viability determination.

Disadvantages:

  • Dye-based.
  • Difficult to differentiate between apoptotic and live cells.

Automated counting

Advantage:

  • Good precision & accuracy.

Disadvantages:

  • Dye-based & sample intensive.
  • Out of focus images result in lost data.

“On-line” counting

Advantage:

  • Automated sampling.

Disadvantages:

  • Space-intensive.
  • Consumes a lot of sample.

“In-line” counting

Advantage:

  • Continuous data and no waste.

Disadvantages:

  • Viability determination is difficult.
  • Cell adherence to probe over time.

SUPPORT

Need any technical help?
Please email us at support@ovizio.com

T: +32 2 600 50 90
E: info@ovizio.com

Ovizio Imaging Systems NV/SA
Rue du Bourdon 100/2
1180 Brussels / Belgium

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