Holography provides the user with an automated, unbiased measurement of cell confluence on the microcarriers, as demonstrated here using Cytodex 1 microcarriers.
The same method can be applied to any round, non-porous, transparent microcarriers.

The OsOne algorithm works in 2 steps:

  • Step 1: process the image to identify the microcarriers
  • Step 2: identify the cells and measure the cell coverage

Step 1: Identification of the microcarriers. Only those centered in the image will be considered for further analysis.

Various levels of confluence on non-porous microcarriers (Cytodex 1 from Cytiva). It can be observed that some microcarriers are completely empty while others are covered with cells, to the point of sticking to each other.

The image above shows various levels of cell coverage on microcarriers. Some are empty, some are colonized, and some are fully covered. It also demonstrates how some microcarriers can become joined together by the cells.

Microcarriers are marked in red by the algorithm.

Step 2: Cell identification and coverage measurement

Holography allows us to capture the signal of the cells, at various confluences.