Philip Mathuis was selected by the International Society for Cell Therapy (ISCT) to lead a discussion group at their annual congress taking place in Paris. The discussion adressed the use of innovative label-free quantitive imaging for automated process control. The session followed a workshop entitled “Introduction to the Benefits and Pitfalls of Multi-Colour Flow Cytometry and Cell Analysis”. The aim of the Workshop was to give an introduction and update of recent flow-cytometry and cell analysis technologies, with particular emphasis on Quality Control and setup for cell therapy applications.