ISCT 2020

Accelerating Cell and Gene Therapy Adoption: From Proof of Concept to Standard of Care

Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration

Animal Cell Technology Unit, iBET, Instituto de Biologia Experimental e Tecnológica
Daniel A. M. Pais , Paulo R. S. Galrão, Anastasiya Kryzhanska, Jérémie Barbau, Inês A. Isidro and Paula M. Alves*

This work shows that DHM can be implemented for online monitoring of Sf9 concentration and viability, as well as product titer (AAV) or culture progression in lytic systems. Therefore, it is a valuable tool to support the time of harvest decision and establishing controlled feeding strategies.

The insect cell-baculovirus vector system has become one of the favorite platforms for the expression of viral vectors for vaccination and gene therapy purposes. Because it is a lytic system, it is essential to balance maximum recombinant product expression with harvest time, minimizing product exposure to detrimental proteases. With this in mind, new bioprocess monitoring solutions are needed to accurately estimate culture progression. Herein, we used online digital holographic microscopy (DHM) to monitor bioreactor cultures of Sf9 insect cells


Ovizio partners with Biocommander to expand and commercialize the iLine F microscope in Greater China

Ovizio Imaging Systems, an innovative microscopy company specializing in automated cell culture monitoring systems, together with Biocommander International, the cell & gene therapy solutions provider in Greater China, today announced that they will enter into a distribution agreement for the supply of iLine F microscopes in Greater China.


SARS-CoV-2 update

Due to the different measures taken by governments all over the world all conferences have been cancelled and or rescheduled to a later date.

We will keep you updated of our new conference schedule as soon as possible.

Stay home and stay healthy

Machine Learning explained

Find out how our flexible machine learning software can be tailored to suit your unique cell culture requirements. OsOne can differentiate between cell types or populations and powers all of our digital holographic microscopes.

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Examination of vesicular stomatitis virus-induced morphology changes in individual Vero cells by qMod microscopy

Viral vectors are increasingly gaining importance in vaccine development, gene therapy and as oncolytic vectors. Vesicular stomatitis virus (VSV), an enveloped virus carrying a negative-sense RNA genome, has proven to be an excellent vaccine vector candidate against infectious diseases and specific cancers.

A variety of assays are available to determine yield of physical particles as well as infectious particles. However, several of the most well-characterized methods, such as plaque assays and tissue culture (TC) infectious dose 50 (TCID50) assays, are associated with a wide variance and are also time- and labor-intensive. Therefore, rapid and reliable means of assaying virus products are welcome advances, especially if the assay easily adapts to multiple product lines.

In this report, we describe a novel viral infection analysis method using a live VSV-based Lassa virus (LASV) vaccine candidate. The recombinant VSV has been genetically altered to express the LASV Josiah glycoprotein (VSVΔG/LASVGP), and infection in Vero cells was examined by microscopy using the Ovizio qMod camera and OsOne software. We assessed characteristics of infected Vero cells over the course of infection, obtained feature measurements of individual cells and examined very early biophysical distinctions of infected cells.

Process Development, Ology Bioservices, Alachua, FL 32615, USA
Isabel Scholz*, Christopher Montoya & Eric Vela


3rd Annual Cell & Gene Therapy Innovation Leaders Summit 2020 in Berlin on Feb. 5 & 6

Ovizio will be present at the 3rd Annual Cell & Gene Therapy Innovation Leaders Summit 2020 in Berlin on Feb. 5 & 6. Feel free to reach out to our CEO, Emilie Viey, who will present the latest results on inline CAR-T cell culture monitoring or to join the talk of Dr. Igor Andreyev (UCL, UK) on Thursday morning. He will share his results on label free differentiation of activated versus non activated human T cells, achieved with the help of Ovizio's qMod, holographic camera, and its associated machine learning platform.

Go to for more info