Jérémie Barbau, Annick Brandenburger, Marissa Nasshan, Jan Van Hauwermeiren

At Ovizio, we are frequently asked why we chose double digital differential holography microscopy (D3HM) to monitor cell viability. We thought that the most comprehensive way to approach an analytical procedure was to question every aspect of it as a validation method. We asked ourselves: What do we want to measure? How can we measure it? How can this be achieved accurately and precisely? What are the techniques involved? Which one is the best? By reading the results of our study, you will get:

  • A clear understanding of the meaning of accuracy and precision in cell viability
  • An overview of the principles of the different methods, their advantages and limits

An insight comparison of the techniques and instrument monitoring in cell viability in terms of their accuracy and precision